To isolate volatile components of a mixture Gas Chromatography is used. It depends on the difference in the mode of partitioning between a flowing mobile phase and a stationary phase. GC has earned its place as one of the most important tools in the industry due to its simplicity, sensitivity, and effectiveness. A sample is vaporized and injected into the separation column's head of the GC system, which is either filled with a finely split solid or covered with a liquid film. The components of a sample are separated as it moves along the column due to variations in how they interact with the stationary phase when an inert gas is used as the mobile phase.
The gas Chromatography Column involves a sample being vapourised and injected onto the head of the chromatographic column. By the flow of inert, gaseous mobile phase sample is transported through the column. The liquid stationary phase is in the column which is absorbed onto the surface of an inert solid.
How to choose Capillary GC Columns?
With the column, an optimized chromatographic separation begins. There are four significant factors for the selection of the proper capillary column for any application.
• Stationary phase
In selecting a column one of the most important step is to choose a stationary phase because it dictates selectivity or the ability of the column to separate sample components. A stationary phase should be chosen based on the application that will be carried out. A stationary phase is a film that is chemically attached to or coated on the inner wall of a Capillary GC Column. The separation procedure is based on the variations in the chemical and physical characteristics of the injected organic molecules and their interactions with the stationary phase. One molecule is kept longer than the other when the analyte-phase interactions between the two compounds are significantly different.
• Column I.D.
Efficiency (number of theoretical plates) and sample capacity can both be balanced by utilising the current range of commercially available capillary column internal diameters (I.D.s) (amount of any one sample component that can be applied to the column without causing the desired sharp peak to overload). One of these needs to be sacrificed to improve the other. The optimum I.D. depends on the analytical requirements for a particular application. Chromatographic efficiency is seen as sharp, well-defined peaks. Capacity increases as column I.D. increases.
• Film Thickness
The optimal film thickness may be different depending on the application. The majority of 0.25 mm I.D. columns have a film thickness of 0.25 or 0.50 M.Sharper peaks (which could boost resolution) and fewer column leaks are advantages of reducing film thickness. These two advantages raise the signal-to-noise ratio. The maximum working temperature of the column will also rise. Reduced analyte capacity and higher analyte contact with the tube wall are disadvantages. Moreover, a thinner film permits analytes to elute at lower temperatures and with shorter retention durations.
Less analyte-tubing interaction and greater sample capacity are advantages. The disadvantages include higher peak widths, which could lower resolution, higher column bleed, and a lower column maximum working temperature. Along with increasing resolution (especially for molecules with low k), increasing film thickness also increases analyte retention and elution temperature.
GC equipment supplier
GC equipment suppliers use several types of detectors including a flame ionization detector, a thermal conductivity detector, a flame photometric detector, an electron capture detector, and a mass spectrometer. GC equipment suppliers provide this powerful standalone application used in many industries. A gas chromatograph should have quick run times and reliable data collecting and analysis software.
